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SRX6453220: Metagenome sequencing of perchlorate-reducing mixed culture CAL
1 ILLUMINA (Illumina MiSeq) run: 3M spots, 895M bases, 519.6Mb downloads

Design: Genomic DNA was extracted using a MoBio PowerSoil DNA Extraction Kit with a cell lysis protocol consisting of vortexing and heating at 70 C for 5 min, repeated twice (MoBio Laboratories, Inc., Carlsbad, CA). DNA library preparation and DNA sequencing were performed by the Adam Arkin Laboratory or the Vincent J. Coates Genomics Sequencing Laboratory at the California Institute of Quantitative Biosciences (QB3, Berkeley, CA)
Submitted by: University of California, Berkeley
Study: Genome-resolved metagenomics of marine perchlorate-reducing communities
show Abstracthide Abstract
Metabolic interactions and genetic exchange are important features of microbial communities. We performed genome-resolved metagenomics to characterize communities performing dissimilatory perchlorate reduction, a metabolism that involves reactive chlorine species and horizontal gene transfer of a conserved functional gene cluster. Distinct communities from source marine sediment and enrichments of varying salinity were recovered, and enriched populations included perchlorate-reducing bacteria as well as putative interacting populations of chlorate-reducing, sulfur-reducing, and fermentative microorganisms. Vectors for perchlorate reduction genes and a novel gene cluster involved in perchlorate reduction were also identified.
Sample: CAL mixed culture
SAMN11978088 • SRS5106562 • All experiments • All runs
Library:
Name: CAL
Instrument: Illumina MiSeq
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 3M spots, 895M bases, 519.6Mb
Run# of Spots# of BasesSizePublished
SRR96948952,994,475895M519.6Mb2019-07-16

ID:
8549554

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